Fractionation of Sera
نویسندگان
چکیده
Sephacryl S-300 has been compared with Sephadex G-200 in the fractionation of sera for the detection of rubella-specific IgM. No difference in sensitivity was found with sera that had low titres of rubella-specific IgM. Sephadex G-200 was apparently more sensitive with some sera which had high titres of rubella-specific IgM due to the contribution of rubella-specific IgA. Sephacryl S-300 offers considerable advantages in ease and speed of preparation, and as it is a more rigid gel higher flow rates can be obtained. Sera may be fractionated in 2 5 hours on Sephacryl S-300 compared with 7-10 hours on Sephadex G-200.
منابع مشابه
Ccliii. Fractionation of Antithyro- Tropic and Antigonadotropic Sera by Charles Robert Harington and Idwal
TEE rapidly growing literature on "antihormones" contains little reference to the fractionation of antithyrotropic or antigonadotropic sera. Thompson [1937] states that the whole activity of antigonadotropic serum is contained in the pseudoglobulin fraction. He gives few details however and the question of the precise distribution of antihormonal activity in such sera remains undecided. The obj...
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